Polymalic acid based nanoconjugates for imaging
AssigneeCedars-Sinai Medical Center
InventorsKeith L. Black, Julia Ljubimova, Alexander Ljubimov, Eggehard Holler
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Abstract
Nanoconjugates that include a polymalic-based molecular scaffold with one or more imaging moiety and one or more targeting modules attached to the scaffold are provided. Methods of targeting a diseased cell or a diseased tissue in a subject by administering the nanoconjugate are described. Methods of synthesizing the nanoconjugate are also provided.
Claims43
A nanoconjugate comprising a polymalic acid-based molecular scaffold, at least one imaging moiety and at least one targeting module, wherein one or more of the at least one imaging moiety is conjugated to the polymalic acid-based molecular scaffold and one or more of the at least one targeting module is conjugated to the polymalic acid-based molecular scaffold.
A kit for facilitating imaging of a cell or a tissue in a subject comprising a nanoconjugate including a polymalic acid-based molecular scaffold, at least one imaging moiety, and at least one targeting module, wherein one or more of the at least one imaging moiety is conjugated to the polymalic acid-based molecular scaffold and one or more of the at least one targeting module is conjugated to the polymalic acid-based molecular scaffold.
A method of targeting a cell or a tissue in a subject comprising administering to the subject a composition including a polymalic acid-based molecular scaffold, at least one imaging moiety, and at least one targeting module, wherein one or more of the at least one imaging moiety is conjugated with the polymalic acid-based molecular scaffold, and one or more of the at least one targeting module is conjugated to the polymalic-acid based molecular scaffold.
A method of synthesizing a nanoconjugate, comprising:
- providing a polymalic acid having a plurality of pendant carboxyl groups;
- reacting a compound containing sulfhydryl groups and amino acid groups through the pendant carboxyl groups to add sulfhydryl groups to the polymalic acid to form an activated polymalic acid;
- reacting at least one imaging moiety containing a sulfhydryl binding group to the activated polymalic acid to form a preconjugate;
- and
- reacting at least one targeting module containing a sulfhydryl binding group to the preconjugate.
Dependencies are inferred from the claim text.