Back to results
US 2012/0258046 A1Published application
Mannose-containing solution for lyophilization, transfection and/or injection of nucleic acids
OwnerThorsten Mutzkeinventor
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
OwnerThorsten Mutzkeinventor
Estimating the term…
The present invention is directed to (the use of) a solution containing at least one nucleic acid (sequence) and free mannose for lyophilization, transfection and/or injection, particularly of RNA and mRNA. The inventive solution exhibits a positive effect on stabilization of the nucleic acid (sequence) during lyophilization and storage but also leads to a considerable increase of the transfection efficiency of a nucleic acid. It thus also increases in vivo expression of a protein encoded by such a nucleic acid upon increased transfection rate. The present invention is furthermore directed to a method of lyophilization using the mannose-containing solution, to pharmaceutical compositions, vaccines, kits, first and second medical uses applying such a mannose-containing solution and/or a nucleic acid (sequence) lyophilized or resuspended with such a solution.
A method of using a solution, wherein the solution comprises at least one nucleic acid sequence and a free, unconjugated and non-covalently bound mannose for lyophilization, transfection and/or injection of the nucleic acid sequence, wherein the solution enhances the transfection efficiency of the nucleic acid and/or the expression of a protein encoded by the nucleic acid sequence.
A solution comprising at least one nucleic acid sequence and a free, unconjugated and non-covalently bound mannose for lyophilization, transfection and/or injection of the nucleic acid sequence, wherein the solution enhances the transfection efficiency of the nucleic acid and/or the expression of a protein encoded by the nucleic acid sequence.
Dependencies are inferred from the claim text.