US 2012/0256102 A1 · Dye-conjugated dendrimers

Abstract

The invention provides dendrimers, conjugates thereof, and methods of using dendrimer conjugates. In one embodiment, the invention provides novel polymeric dendrimers as a new class of fluorescent labels. The labels can include multiple fluorescent dye molecules conjugated to a single polymeric backbone or core, such as a dendrimer. The dendrimers can have regular or irregular branched polymeric network structures that allow for the chemical attachment of multiple dye molecules, multiple color dyes, and/or multiple functional groups, in a combinatorial fashion. The fluorescent dendritic nanoprobes (FDNs) thus provide a new class of fluorescent reporters for fluorescence microscopy and imaging.

Claims21

Dependent claims

A dendrimer of Formula I:

wherein
the circle of Formula I is a G2-G10 dendrimer;
each R is independently a dye moiety covalently bonded to a branch of the dendrimer;
each linker-X is independently the same or different, wherein linker is S, NH, or O, or a chain of 2-40 atoms that connects a branch of the dendrimer to a group X;
each X is independently R, a reactive group, an affinity label, or a targeting moiety;
n is a value representing about 1% to about 50% of terminal dendrimer groups;
m is a value representing 0% to about 50% of terminal dendrimer groups;
s is a value representing 0% to about 60% of terminal dendrimer groups;
and
t is a value representing 0% to about 60% of terminal dendrimer groups;
wherein at least two R groups are different from each other;
and
the diameter of the dendrimer is less than about 20 nm;
or a salt thereof.

11 more elements

Dependencies are inferred from the claim text.

Dye-conjugated dendrimers

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Dye-conjugated dendrimers

Life of this patent

Abstract

Claims21