Super-resolution microscopy system using speckle illumination and array signal processing
AssigneeKorea Advanced Institute of Science and Technology
InventorsJong Chul Ye, Jae Duck Jang
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Abstract
A nano-scale resolution fluorescence microscopy system and a method of obtaining an image using the nano-scale resolution microscopy system, and more particularly, a method and a microscopy system, capable of observing fluorescence probes in high resolution by radiating an irregular diffused light to have an incoherent speckle pattern that has low correlation in an adjacent space are disclosed. According to embodiments of the present invention, a diffraction limit of a fluorescence microscope may be overcome, and a super high resolution image on a nanometer scale may be obtained.
Claims26
A nano-scale resolution fluorescence microscopy system, comprising:
- a speckle pattern generator to generate speckle patterns by passing, through a diffuser, a light emitted from at least one coherent light source in at least one color to detect fluorescent emission in at least one color from fluorophores attached to a specimen;
- an illumination optical system to radiate the generated speckle patterns to be exposed on a specimen, by disposing at least one optical lens;
- and
- a fluorescent image optical system to detect fluorescence emission images of the specimen excited by the speckle patterns in at least one color, by passing the radiated speckle patterns sequentially through at least one excitation filter and detecting the emission images through at least one emission filter.
A method of obtaining an image using a nano-scale resolution microscopy system, the method comprising:
- illuminating coherent light from at least one coherent source to image fluorphores in specimen;
- generating speckle patterns by passing the light emitted from the coherent through a diffuser
- radiating the generated speckle patterns to be exposed on a specimen, using at least one optical lens;
- and
- photographing a fluorescent light of the speckle patterns, by passing the radiated speckle patterns sequentially through at least one excitation filter and at least one emission filter.
A nano-scale resolution microscopy system, comprising:
- an incident optical system;
- a fluorescent image optical system;
- a speckle pattern generator to generate speckle patterns by passing, through a diffuser or a spatial light modulator, a light emitted from a laser to photograph fluorescence emission from a specimen;
- and
- an image reconstruction algorithm execution unit to execute an image reconstruction algorithm,
- wherein the incident optical system and the fluorescent image optical system include at least one optical lens disposed in a structure of at least one of a reflection microscope, a transmission microscope, and a total internal reflection optical microscope, and
- the image reconstruction algorithm execution unit executes the image reconstruction algorithm, thereby continuously obtaining images photographed by the fluorescent image optical system, and reconstructing the images in high resolution by sensing time dependent data that changes in every pixel, based on the continuously obtained images.
Dependencies are inferred from the claim text.