Plasmid for expression of transgenes in plants

A plasmid comprising segments operationally configured adjacent to each other in the following order:

• a binary vector comprising a broad-host-range replication origin, a gene encoding antibiotic resistance in bacteria, T-DNA border repeats configured for mediating transfer of DNA from Agrobacterium tumefaciens into a selected plant, and a multiple cloning site positioned between the T-DNA border repeats;
• an upstream homologous region for targeting, together with a downstream homologous region, incorporation of DNA transferred from Agrobacterium tumefaciens into the genome of the selected plant;
• a first promoter for promoting transcription of a first adjacent coding sequence;
• the first adjacent sequence;
• a first terminator for signaling termination of transcription of the first adjacent sequence;
• a second promoter for promoting transcription of a second adjacent sequence;
• the second adjacent sequence;
• a second terminator for signaling termination of transcription of the second adjacent sequence;
• and
• the downstream homologous region for targeting, together with the upstream homologous region, incorporation of DNA transferred from Agrobacterium tumefaciens into the genome of the selected plant.