Treatment of Alopecia By Micropore Delivery of Stem Cells

A method of treating or preventing hair loss in a subject in need thereof, the method comprising:

• irradiating skin with pulses of laser irradiation at a rate of 10 per second to 5000 per second and at a pulse energy of up to 150 mJ per pulse to form a plurality of micropore channels, wherein the micropore channels extend into dermis of the skin;
• and
• implanting a composition into the micropore channel,
• wherein the composition comprises stem cells and a growth media, the laser irradiation has an absorption coefficient in water of about 100 cm−1 to 12,300 cm−1, the micropore channels have a depth of about 200 μm to 4 mm and a density of less than 2500 voids per cm2 per pass, and viable tissue separating adjacent micropore channels has a width, at a narrowest point thereof, between about 50 μm and 500 μm.