Production method of 11-sugar sialylglycopeptide
AssigneeThe Noguchi Institute
InventorsShuichi Sugawara, Kenji Osumi
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Abstract
It is an object of the present invention to provide a method for producing an 11-sugar sialylglycopeptide easily and with good yield and a high degree of purity on an industrial scale from defatted bird egg yolks. The present invention provides a production method of an 11-sugar sialylglycopeptide. More specifically, the present invention provides a production method of an 11-sugar sialylglycopeptide comprising: an extraction step of extracting defatted bird egg yolks with water or a salt solution to obtain a liquid extract of a glycopeptide, a precipitation step of adding the liquid extract to a water-soluble organic solvent to precipitate the glycopeptide, and a desalting step of desalting the precipitate.
Claims16
A production method of an 11-sugar sialylglycopeptide, comprising:
- an extraction step of extracting defatted bird egg yolks with water or a salt solution to obtain a liquid extract of a glycopeptide,
- a precipitation step of adding the liquid extract to a water-soluble organic solvent to precipitate the glycopeptide, and
- a desalting step of desalting the precipitate.
A production method of an 11-sugar sialyiglycopeptide, comprising:
- an extraction step of extracting defatted bird egg yolks with water to obtain a liquid extract of a glycopeptide,
- a precipitation step of adding the liquid extract to ethanol to precipitate the glycopeptide, and
- a desalting step of desalting the precipitate with ODS resin.
A production method of an 11-sugar sialylglycopeptide, comprising:
- an extraction step of extracting defatted bird egg yolks with water to obtain a liquid extract of a glycopeptide,
- a precipitation step of adding the liquid extract to ethanol to precipitate the glycopeptide, and
- a step of retaining the precipitate on ODS resin, washing the precipitate on the ODS resin with water, and then eluting the glycopeptide with an aqueous organic solvent.
Dependencies are inferred from the claim text.