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US 2012/0238477 A1Published application
Methods for synthesis of an oligopeptide microarray
AssigneeRoche Nimble Gen, Inc.
InventorsTom Albert, Todd Richmond, Matthew Rodesch, Klause-Peter Stengele, Jochen Bühler, Markus Ott
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Abstract
An oligopeptide microarray and methods for the synthesis thereof are presented. Further presented is a microarray on a solid support comprising at least about 10,000 oligopeptide features per cm2 and preferably at least about 50,000 oligopeptide features per cm2.
Claims44
A method for synthesizing an oligopeptide microarray, wherein the method comprises the steps of:
- a) coupling to a reactive amino group attached directly or indirectly to a surface of a plastic solid support an amino acid comprising an amino group protected by a photolabile moiety selected from 2-(2-nitrophenyl)-propoxycarbonyl (NPPOC) and a derivative thereof with the proviso that the photolabile moiety is a benzoyl NPPOC derivative when the amino acid is tyrosine;
- b) optionally capping unreacted amino acids;
- c) optionally washing the plastic solid support;
- d) photoirradiating the protected amino group such that the photoirradiated amino group is reactive;
- and
- e) repeating steps b) to d) for a predetermined number of times, whereby an oligopeptide is synthesized.
3 more elements
A method for synthesizing an oligopeptide microarray, wherein the method comprises the steps of:
- a) coupling to a reactive amino group attached directly or indirectly to a surface of a solid support an amino acid comprising an amino group protected by a photolabile moiety selected from 2-(2-nitrophenyl)-propoxycarbonyl (NPPOC) and a derivative thereof with the proviso that the photolabile moiety is a benzoyl NPPOC derivative when the amino acid is tyrosine;
- b) optionally capping unreacted amino acids;
- c) optionally washing the solid support;
- d) photoirradiating the protected amino group such that the photoirradiated amino group is reactive;
- and
- e) repeating steps b) to d) for a predetermined number of times, whereby an oligopeptide is synthesized.
3 more elements
Dependencies are inferred from the claim text.