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US 2012/0238475 A1Published application
Methods of Amplifying and Sequencing Nucleic Acids
OwnerJohn H. Leamon, Kenton L. Lohman +2inventors
InventorsJohn H. Leamon, Kenton L. Lohman, Jonathan M. Rothberg, Michael P. Weiner
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Abstract
An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
Claims21
1) A system for sequencing nucleic acids, comprising:
- a perfusion chamber comprising an upper side and an array of at least 10,000 reaction chambers a plurality of which comprise a bead each with multiple clonal copies of a species of nucleic acid immobilized thereon, wherein the sealed perfusion chamber further comprises a first port detachably coupled to a fluidics subsystem that comprises a single inlet conduit operatively coupled to a manifold, and an A nucleotide tube, a G nucleotide tube, a T nucleotide tube, and a C nucleotide tube.
Dependencies are inferred from the claim text.