Exosome-associated microrna as a diagnostic marker
AssigneeUniversity of Louisville Research Foundation, Inc.
InventorsDouglas D. Taylor, Cicek Gercel-Taylor
Life of this patent
Estimating the term…
Abstract
The presently disclosed subject matter provides methods of diagnosis of cancer or adverse pregnancy outcomes in a subject by measuring amounts of one or more microRNAs present in cancer-derived exosomes isolated from a biological sample from the subject.
Claims12
A method for assessing the presence of one or more biomarkers from microvesicles, comprising isolating a population of extracellular microvesicles from a biological sample using a microvesicle surface marker, isolating microRNA from said population of microvesicles, and determining a presence of one or more microRNA in said isolated microRNA.
A method of determining a microRNA expression profile of microvesicles, comprising:
- isolating a population of extracellular microvesicles from a biological liquid sample;
- isolating microRNA from said isolated microvesicles and determining an expression profile of one or more microRNA in said isolated microRNA;
- and
- comparing the expression profile with a profile from a reference sample to determine the microRNA expression profile for said microvesicles.
Dependencies are inferred from the claim text.