Storage-stable liquid washing or cleaning agent containing proteases

Liquid washing or cleaning agent comprising:

• (a) a protease chosen from
• a. a protease comprising an amino acid sequence according to SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7 or SEQ ID NO. 8;
• b. a protease comprising, relative to SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, or SEQ ID NO. 8, an amino acid sequence modified in at least one position, the modification in the numbering according to SEQ ID NO. 1 being chosen from:
• i. threonine in position 3 (3T),
• ii. isoleucine in position 4 (4I),
• iii. alanine, threonine or arginine in position 61 (61A, 61T or 61R),
• iv. aspartic acid or glutamic acid in position 154 (154D or 154E),
• v. proline in position 188 (188P),
• vi. methionine in position 193 (193M),
• vii. isoleucine in position 199 (199I),
• viii. aspartic acid, glutamic acid or glycine in position 211 (211D, 211E or 211G),
• ix. combinations of amino acids (i) to (viii);
• and
• (b) a phosphonate.

Method of providing proteolytic activity in a liquid washing or cleaning agent comprising adding to the agent a protease,

• (a1) having an amino acid sequence which is at least 80% identical to the amino acid sequence stated in SEQ ID NO. 1 and comprising in position 99 in the numbering according to SEQ ID NO. 1 the amino acid glutamic acid (E) or aspartic acid (D), or
• (a2) having an amino acid sequence which is at least 80% identical to the amino acid sequence stated in SEQ ID NO. 1 and comprising in position 99 in the numbering according to SEQ ID NO. 1 the amino acid asparagine (N) or glutamine (Q), or
• (a3) having an amino acid sequence which is at least 80% identical to the amino acid sequence stated in SEQ ID NO. 1 and comprising in position 99 in the numbering according to SEQ ID NO. 1 the amino acid alanine (A) or glycine (G) or serine (S), and
• (b) a phosphonate.

Method of providing proteolytic activity in a liquid washing or cleaning agent comprising adding to the agent a protease chosen from:

• (a) a protease comprising an amino acid sequence according to SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, or SEQ ID NO. 8;
• (b) a protease comprising, relative to SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, or SEQ ID NO. 8, an amino acid sequence modified in at least one position, the modification in the numbering according to SEQ ID NO. 1 being chosen from:
• i. threonine in position 3 (3T),
• ii. isoleucine in position 4 (4I),
• iii. alanine, threonine or arginine in position 61 (61A, 61T or 61R),
• iv. aspartic acid or glutamic acid in position 154 (154D or 154E),
• v. praline in position 188 (188P),
• vi. methionine in position 193 (193M),
• vii. isoleucine in position 199 (199I),
• viii. aspartic acid, glutamic acid or glycine in position 211 (211D, 211E or 211G),
• ix. combinations of amino acids (i) to (viii);
• wherein the liquid washing or cleaning agent further comprises a phosphonate.