Production of viral vaccines in suspension on avian embryonic derived stem cell lines
AssigneeVivalis
InventorsMajid Mehtali, Patrick Champion-Arnaud, Arnaud Leon
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Abstract
The present invention relates to the development and manufacturing of viral vaccines. In particular, the invention relates to the field of industrial production of viral vectors and vaccines, more in particular to the use of avian embryonic stem cells, preferably the EBx® cell line derived from chicken embryonic stem cells, for the production of viral vectors and viruses. The invention is particularly useful for the industrial production of viral vaccines to prevent viral infection of humans and animals.
Claims20
A process of production of influenza virus which comprises replicating an influenza virus in avian embryonic derived stem cells EBx®, said process comprising the steps of:
- a) proliferating said EBx® cells in a cultivation vessel, in suspension, in a serum-free medium No 1;
- b) infecting the cells with said influenza virus when the cell density is of at least 1.5 million cells/ml;
- c) shortly before infection, simultaneously to infection, or shortly after infection adding to the cell culture serum-free medium No 2;
- and
- d) further culturing said infected cells in order to allow virus replication;
- and
- e) optionally, harvesting said virus.
An avian embryonic derived stem cell EBx® that is infected with an influenza virus.
Use of avian embryonic derived stem cells EBx® to generate and produce influenza virus re-assorted viruses.
Dependencies are inferred from the claim text.