Conjugation Reactions
AssigneeInnova Biosciences Limited
InventorsNicholas Gee, Annamaria Draghi
Life of this patent
Estimating the term…
Abstract
We describe methods that allow either carbodiimides or other carboxyl-reactive substances to be mixed with solutions of carboxylic acids or phosphates or amines or combinations thereof, so as to form a homogeneous mixture which is then dried, preferably in a freeze drying process. The mixture is then contacted with an entity, which preferably involves the dissolution of the mixture with a buffered solution of the entity, so as to initiate a conjugation reaction between the entity and a component in the mixture.
Claims20
A method of producing a reagent for use in a conjugation reaction between a first reactant containing a carboxyl group or a phosphate group and a second reactant containing an amine group, comprising
- a) forming a solution or suspension of
- i) a carboxyl-activating or phosphate-activating non-enzymatic substance capable of acting on a carboxyl group- or phosphate group-containing moiety to facilitate the formation of an amide bond between a carboxyl group and an amine group or a phosphoramidate bond between a phosphate group and an amine group, and
- ii) a first reactant or a second reactant;
- and
- b) drying the solution or suspension.
A dried reagent for use in a conjugation reaction between a first reactant containing a carboxyl group or a phosphate group and a second reactant containing an amine group, comprising a homogeneous mixture of i) a carboxyl-activating or phosphate-activating non-enzymatic substance capable of acting on a carboxyl group or phosphate group-containing moiety to facilitate the formation of an amide bond between a carboxyl group and an amine group or a phosphoramidate bond between a phosphate group and an amine group, and ii) a first reactant or a second reactant.
Dependencies are inferred from the claim text.