Method for the biocatalytic cyclization of terpenes and cyclase mutants employable therein
AssigneeBASF SE
InventorsMICHAEL BREUER, BERNHARD HAUER, DIETER JENDROSSEK, GABRIELE SIEDENBURG, JUERGEN PLEISS, DEMET SIRIM, SILVIA RACOLTA
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Abstract
The present invention relates to novel mutants with cyclase activity and use thereof in a method for biocatalytic cyclization of terpenes, such as in particular for the production of isopulegol by cyclization of citronellal; a method for the preparation of menthol and methods for the biocatalytic conversion of further compounds with structural motifs similar to terpene.
Claims27
1-26. (canceled)
An enzyme mutant with cyclase activity which comprises an amino acid sequence selected from the group consisting of the amino acid sequences of SEQ ID NO:
- 2 to 326 or a partial sequence thereof, wherein said enzyme mutant is a mutant of a wildtype enzyme and catalyzes at least the cyclization of a citronellal isomer to at least one isopulegol isomer.
A method of production of isopulegol of general formula (I)
- comprising cyclizing citronellal of general formula (II)
- to isopulegol of formula (I) by means of an enzyme of EC class EC 5.4.99 or EC class EC 5.4.99.17, or in the presence of a microorganism expressing said enzyme.
A method for the cyclization of terpenes, the conversion of citronellal to isopulegol, or the conversion of compounds of the general formula IV, comprising utilizing an enzyme from EC class EC 5.4.99 or EC class EC 5.4.99.17.
Dependencies are inferred from the claim text.