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AssigneeGenomatica, Inc.
InventorsMark J. BURK, Anthony P. Burgard, Priti Pharkya
Estimating the term…
A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.
1-65. (canceled)
A non-naturally occurring eukaryotic organism, comprising one or more gene disruptions, said one or more gene disruptions occurring in genes encoding enzymes imparting increased acrylate production in said organism when said gene disruption reduces an activity of said enzyme, whereby said one or more gene disruptions confers increased production of acrylate onto said organism.
A method for producing acrylic acid, comprising culturing a non-naturally occurring eukaryotic organism, comprising one or more gene disruptions, said one or more gene disruptions occurring in genes encoding enzymes imparting enhanced acrylate production to growth of said organism when said gene disruption reduces an activity of said enzyme, whereby said one or more gene disruptions confers increased production of acrlyate onto said organism.
The method of claim 80 further comprising gene disruptions encoding enzymes selected from the group consisting of a fumarase and a soluble fumarate reductase.
Dependencies are inferred from the claim text.