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US 2012/0237974 A1Published application
Method for the expression of a recombinant protein in a mammalian cell
AssigneeAmarna Holding B.V.
InventorWalter Gerhardus de Vries
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AssigneeAmarna Holding B.V.
InventorWalter Gerhardus de Vries
Estimating the term…
The invention relates to methods for the production of a recombinant protein in a mammalian cell and methods to enhance the production of recombinant proteins in mammalian cells. More in particular, the invention provides a cell for the production of a recombinant protein of interest wherein said cell is permissive to a polyomavirus and wherein said cell comprises the genetic elements A and B wherein A encodes a polyomaviral large T antigen or a functional equivalent thereof and B comprises a gene encoding a protein of interest under the functional control of a polyomaviral origin of replication or a functional equivalent thereof, wherein said cell lacks the capability to express a polyomaviral small T antigen or a functional equivalent thereof as well as the capability to express a polyomavirus capsid protein.
A method for the production of a protein of interest in a mammalian cell permissive to a polyomavirus comprising the genetic elements A and B wherein element A encodes a polyomaviral large T antigen or a functional equivalent thereof and B comprises a gene encoding a protein of interest under the functional control of the polyomaviral origin of replication or a functional equivalent thereof, wherein the cell lacks the capability to express a polyomaviral small T antigen or a functional equivalent thereof as well as the capability to express a polyomavirus capsid protein, the method comprising:
A mammalian cell permissive to a polyomavirus comprising the genetic elements A and B wherein A encodes a polyomaviral large T antigen or a functional equivalent thereof and B comprises a gene encoding a protein of interest under the functional control of the polyomaviral origin of replication or a functional equivalent thereof, wherein the cell lacks the capability to express a polyomaviral small T antigen or a functional equivalent thereof as well as the capability to express a polyomavirus capsid protein.
Dependencies are inferred from the claim text.