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US 2012/0237969 A1Published application
Method of Preparing Cells Susceptible to Transmissible Spongiform Encephalopathy and the Creation of TSE Persistently Infected Cells
AssigneeAnimal, Plant and Fisheries Quarantine and Inspect AGency
InventorsDong-Seob Tark, Hyo-Jin Kim, Hyun-Joo Shon, Yoon-Hee Lee, Min-Jeong Kim, Eun-Im Yun, In-Soo Cho, Chang-Hee Kweon, Yi-Seok Joo, Otto Windl, Michael Neale
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Abstract
Disclosed is a method for preparation of transmissible spongiform encephalopathy (TSE) susceptible cells, and such cells may be efficiently used in diagnosis of TSE, etiology studies and development of novel therapeutics, etc.
Claims6
A method for preparation of a cell susceptible to transmissible spongiform encephalopathy, comprising:
- extracting a normal prion protein gene from the brain of a major natural host of TSE and cloning the same;
- inserting the cloned normal prion protein gene into a lentivirus transfer vector to produce an infectious recombinant lentivirus;
- and,
- inoculating the infectious recombinant lentivirus into a non-human mammalian cell-line to produce a transduced cell expressing a normal prion protein.
1 more element
A method for the preparation of a transmissible spongiform encephalopathy infected cell, comprising:
- extracting a normal prion protein gene from the brain of a major natural host of transmissible spongiform encephalopathy and cloning the same;
- inserting the cloned normal prion protein gene tinto a lentivirus transfer vector to produce an infectious recombinant lentivirus;
- inoculating the infectious recombinant lentivirus into a mammalian cell-line to produce a transduced cell expressing a normal prion protein;
- selecting one of multiple transduced cells, which expresses the normal the normal prion protein;
- inoculating the selected transduced cell expressing the normal prion protein to screen infected cells;
- with transmissible spongiform encephalopathy infected brain tissue and
- cloning the TSE infected cells to confirm the transmissible spongiform encephalopathy infected cell-line.
4 more elements
Dependencies are inferred from the claim text.