Treponema pallidum triplet antigen
AssigneeOrth-Clinical Diagnostics, Inc.
InventorsJian Zheng, Renee Yura, Jianping Yang
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Abstract
A Treponema pallidum triplet antigen construct is disclosed which includes three Treponema pallidum antigens (TP15, TP17, and TP47), as well as a ten amino acid leader sequence (tag 261) and human copper zinc superoxide dismutase (hSOD). This construct is optimized for in vitro diagnosis of syphilis infection. Plasmids containing DNA encoding the triplet antigen, host cells, production methods, detection methods, and kits are also disclosed.
Claims45
A recombinant plasmid encoding a Treponema pallidum triplet antigen, wherein the plasmid comprises nucleic acid encoding an amino acid sequence selected from the group consisting of SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, and SEQ ID NO:12.
A recombinant plasmid encoding a Treponema pallidum triplet antigen, the recombinant plasmid selected from the group consisting of:
- the plasmid designated p261nS-TP17-15-47 and deposited with the American Type Culture Collection as ATCC Accession No. PTA-11590;
- the plasmid designated p261nS-TP47-17-15 and deposited with the American Type Culture Collection as ATCC Accession No. PTA-11589;
- the plasmid designated p261nS-TP17-47-15;
- the plasmid designated p261nS-TP47-15-17;
- the plasmid designated p261nS-TP15-17-47;
- and
- the plasmid designated p261nS-TP15-47-17.
A Treponema pallidum triplet antigen having an amino acid sequence selected from the group consisting of SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, and SEQ ID NO:12.
Dependencies are inferred from the claim text.