Immortalized cell compositions and compositions derived therefrom
OwnerRandall G. Rupp, Linda O. Palladinoinventors
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Abstract
The invention is directed to immortalized cell compositions and compositions derived therefrom. The invention is further directed to methods of making and using such immortalized cell compositions and compositions derived therefrom. Such immortalized cell compositions include but are not limited to Immortalized Amnion-derived Multipotent Progenitor cells (herein referred to as I-AMP cells) and conditioned media derived therefrom (herein referred to as I-Amnion-derived Cellular Cytokine Solution or I-ACCS).
Claims19
A composition comprising a population of Immortalized Amnion-derived Multipotent Progenitor (I-AMP) cells, wherein the I-AMP cells are capable of greater than 12 population doublings.
A method of making I-AMP cells comprising:
- a) isolating amnion epithelial cells from the amnion of a placenta;
- b) selecting AMP cells from the amnion epithelial cells;
- and
- c) manipulating the AMP cells to immortalize them, wherein immortalization is characterized by the I-AMP cells being capable of greater than 12 population doublings.
The method of 4 wherein the viral-induced genetic manipulation is accomplished using EBV, SV40 T antigen, adenovirus, or human papillomavirus.
A method of making an I-Amnion-derived Cellular Cytokine Solution (I-ACCS) comprising
- a) culturing for a first time I-AMP cells until they reach confluence;
- b) changing the culture medium;
- c) culturing for a second time the I-AMP cells;
- and
- d) collecting the culture medium of step (c) to obtain I-ACCS.
Dependencies are inferred from the claim text.