US 2012/0237496 A1 · PROTEASE VARIANTS

Protease variants

OwnerJoerg Birkenfeld, Andrea Eicker +13inventors

InventorsJoerg Birkenfeld, Andrea Eicker, Per-Ola Freskgard, Claudia Gotzberger-Schad, Joanna Grudzinska, Ulrich Haupts, Josi Innig, Christoph Mahlert, Andreas Scheidig, Michael Strerath, Jan Tebbe, Johan Per-Wallin, Nina Wobst, Carl Innes Webster, Lutz Jermutus

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Abstract

The present invention relates to polypeptides comprising protease variants of wild type human neprilysin having an altered specificity and/or activity. In particular the present invention relates to polypeptides comprising protease variants derived from human neprilysin having an increased specificity and/or activity against certain substrates, in particular against amyloid beta.

Claims15

Dependent claims

A polypeptide comprising a protease variant of wild type human neprilysin extracellular catalytic domain (SEQ ID NO:

2), said polypeptide having a greater specificity for an Aβ peptide compared to wild type human neprilysin (SEQ ID NO:
1), wherein G399 is replaced by another naturally occurring amino acid and/or G714 is replaced by another naturally occurring amino acid, optionally said naturally occurring amino acid is other than Ala (A).

Dependencies are inferred from the claim text.

Facts

Application

13/378,764Filed 21 Jun 2010

Priority

19 Jun 2009

Published

20 Sep 2012

Term ends

Not estimatedThe source records don’t support an estimate.

Classification8 codes

C12N 9/6494
Metalloendopeptidases: neprilysin , i.e. enkephalinase or neutral-endopeptidase 24.11
A61P 25/28
Drugs for disorders of the nervous system: for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents,…
C12Y 304/24011
Metalloendopeptidases: neprilysin , i.e. enkephalinase or neutral endopeptidase 24.11
A61K 38/00
Medicinal preparations containing peptides