Nucleic acids encoding a mut-t domain-containing polypeptide
AssigneeBeth Israel Deaconess Medical Center, Inc.
InventorAndrew M. Scharenberg
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Abstract
The invention pertains to nucleic acids encoding a mutT domain-containing polypeptide, including fragments and biologically functional variants thereof. The invention also pertains to therapeutics and diagnostics involving the foregoing polypeptide and nucleic acids and agents that bind the foregoing polypeptide and nucleic acids. The invention also pertains to the identification of a novel mutT domain in human TrpC7, a polypeptide previously described as a putative calcium ion channel. Accordingly, the invention also pertains to methods and compositions for identifying agents useful in modulating mutT domain-mediated calcium or other ion transport in cells expressing a polypeptide comprising a mutT domain and a calcium or other ion channel.
Claims25
1-41. (canceled)
An isolated nucleic acid, comprising:
- (a) a nucleotide sequence which hybridizes under stringent conditions to the complementary sequence of SEQ ID NO:1 and encodes a mutTCCH-1 polypeptide having hydrolase activity, wherein the stringent conditions comprise hybridization at 65° C. in a hybridization buffer comprising 3.5×SSC, 0.02% Ficoll, 0.02% polyvinyl pyrolidone, 0.02% Bovine serum albumin, 2.5 mM NaH2PO4 (pH 7), 0.5% SDS, and 2 mM EDTA, SSC being 0.15M sodium chloride/0.15M sodium citrate, pH 7;
- SDS being sodium dodecyl sulphate, and EDTA being ethylenediaminetetracetic acid;
- (b) a full-length complement of (a), or
- (c) a fragment of (a), wherein the fragment is not identical to any fragment of a sequence selected from the group consisting of the sequences having the database accession numbers of Table I, and the fragment has a size selected from the group consisting of at least 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 50, 75, 100, and 200 nucleotides.
Dependencies are inferred from the claim text.