Systems and methods for autologous biological therapeutics
AssigneeGreyledge Technologies, LLC
InventorsDavid Karli, David L. Bombard
Life of this patent
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Abstract
This disclosure describes systems, methods, and apparatus for forming concentrates of platelet-rich plasma or bone marrow cells having user-defined concentrations, concentration ranges, and/or volumes. Whole blood or bone marrow samples can be passed through one or two separation operations in which platelets or bone marrow cells are separated from red blood cells and concentrated in a plasma. During this separation and concentrating, a total number of platelets or bone marrow cells or a concentration of either is determined and then used to ascertain what volumes and concentrations need be mixed in order to produce a platelet-rich plasma concentrate or a bone marrow-rich plasma concentrate having a target concentration and/or volume.
Claims22
A method of generating a platelet-rich-plasma concentrate or a bone marrow cell-rich plasma concentrate comprising:
- separating a whole blood sample or a bone marrow sample into a red blood cell fraction, a platelet-poor plasma or a bone marrow cell-poor plasma, and a platelet-rich plasma or bone marrow cell-rich plasma;
- determining a platelet concentration or bone marrow cell concentration, before, during, or after the separating, via at least a first measurement;
- determining a first volume of fluid in which the first determining was performed in;
- using the concentration and the first volume to determine a second volume of platelet-poor plasma or bone marrow cell-poor plasma that, when mixed with the platelet-rich plasma or bone marrow cell-rich plasma, will provide a concentration of platelets or bone marrow cells falling within a target concentration range;
- and
- creating a platelet-rich plasma concentrate or bone marrow cell-rich plasma concentrate by mixing the second volume of platelet-poor plasma or bone marrow cell-poor plasma with the platelet-rich plasma or bone marrow cell-rich plasma.
A cell concentrating system comprising:
- a blood separation component having a blood sample input and being configured to separate the blood sample into a red blood cell fraction, and a plasma fraction, where the plasma fraction comprises a target cell-rich fraction and a target cell-poor fraction;
- a measurement system that measures a total number of target cells in the cell concentrating system;
- concentration and flow logic that determines a first volume of the target cell-poor fraction to mix with the target cell-rich fraction in order to form a target cell-rich concentrate having a concentration of target cells that is within a target concentration range;
- and
- a mix component configured to form the target cell-rich concentrate by mixing the first volume of the target cell-poor fraction with the target cell-rich fraction.
A method of generating a target cell-rich concentrate comprising:
- separating a target blood sample into a red blood cell fraction, a target cell-poor fraction, and a target cell-rich fraction;
- determining a total number of target cells in the target blood sample;
- calculating a first volume of the target cell-poor fraction that, when mixed with the target cell-rich fraction, will provide a concentration of target cells within a target concentration range;
- and
- creating a target cell-rich concentrate by mixing the first volume of the target cell-poor fraction with the target cell-rich fraction.
Dependencies are inferred from the claim text.