Novel molecular beacons
AssigneeThe Trustees of the University of Pennsylvania
InventorsAntony Kuang-Shih Chen, Andrew Tsourkas
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Abstract
The invention provides novel compositions and methods for the detection of a target molecule. Specifically, the invention provides a bimolecular beacon composition comprising two nucleic acid molecules, and methods thereof. One of the nucleic acid molecule is operably linked to a reporter detectable label and the other nucleic acid molecule is operably linked to a reference detectable label and a quencher. In some embodiments, at least one of the nucleic acid molecules comprises a single stranded overhang.
Claims62
A bimolecular beacon comprising:
- a first nucleic acid operably linked to a first detectable label at one of its ends;
- and
- a second nucleic acid operably linked to a second detectable label at its one end and a quencher at its other end.
A composition comprising:
- a first nucleic acid operably linked to a first detectable label at one of its ends and a second nucleic acid operably linked to a second detectable label at its one end and a quencher at its other end.
An oligonucleotide probe comprising:
- a first nucleic acid operably linked to a first detectable label at one of its ends and a second nucleic acid operably linked to a second detectable label at its one end and a quencher at its other end.
A kit comprising:
- a first nucleic acid operably linked to a first detectable label at one of its ends and a second nucleic acid operably linked to a second detectable label at its one end and a quencher at its other end.
A method for detecting a polymorphism associated with target gene comprising:
- providing a molecular beacon comprising a first nucleic acid operably linked to a first detectable label at one of its ends and a second nucleic acid operably linked to a second detectable label at its one end and a quencher at its other end;
- detecting said first and second detectable labels;
- and
- determining a relative fluorescent intensity of said first and second detectable labels, whereby the relative intensities indicate the presence or absence of said polymorphism, thereby detecting said polymorphism.
A method for detecting the expression level of a target gene comprising:
- providing a molecular beacon comprising a first nucleic acid operably linked to a first detectable label at one of its ends and a second nucleic acid operably linked to a second detectable label at its one end and a quencher at its other end;
- detecting said first and second detectable labels;
- and
- determining a relative fluorescent intensity of said first and second detectable labels, whereby the relative intensities indicate the expression level of said target gene, thereby detecting the expression level of said target gene.
A method for diagnosis of a disease in a subject comprising:
- providing a molecular beacon comprising a first nucleic acid operably linked to a first detectable label at one of its ends and a second nucleic acid operably linked to a second detectable label at its one end and a quencher at its other end;
- detecting said first and second detectable labels;
- and
- determining a relative fluorescent intensity of said first and second detectable labels, whereby the relative intensities indicate the presence or absence of said disease in said subject, thereby diagnosing said disease.
A method for monitoring a transfection efficiency comprising:
- providing a molecular beacon comprising a first nucleic acid operably linked to a first detectable label at one of its ends and a second nucleic acid operably linked to a second detectable label at its one end and a quencher at its other end;
- detecting said first and second detectable labels;
- and
- determining a relative fluorescent intensity of said first and second detectable labels, whereby the relative intensities indicate said transfection efficiency.
A bimolecular beacon comprising:
- a first nucleic acid operably linked to a detectable label;
- and
- a second nucleic acid operably linked to a quencher.
Dependencies are inferred from the claim text.