Differentiation of flow cytometry pulses and applications
AssigneeEMD Millipore Corporation
InventorsRichard Pittaro, Bruce Goldman, Ray Lefebvre, David A. King
Life of this patent
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Abstract
A method of analyzing pulses from a flow cytometer in which particles in a fluid pass through an excitation volume of an electromagnetic radiation and interact with the electromagnetic radiation to generate signals in the form of pulses includes generating a time-dependent pulse indicative of the characteristics of one or more particles passing through the excitation volume of the electromagnetic radiation, determining a measurement window by selecting a portion of the pulse with a starting point and an ending point above a predetermined value, and calculating a first derivative of the pulse with respect to time over the measurement window.
Claims4
A method of tracking particles in a multi-source capillary flow cytometer comprising:
- providing a first and a second excitation volumes using a first and a second light beams respectively;
- passing a fluid containing particles through the first excitation volume thereby generating a first time-dependent pulse;
- computing a first first derivative of the first time-dependent pulse with respect to time;
- calculating a first velocity of the particle using the computed first first derivative of the first time-dependent pulse;
- and
- determining an arrival time of the particle at the second excitation volume using the calculated first velocity of the particle.
Dependencies are inferred from the claim text.