Use of pon gene cluster in preparing medicament for treating atherosclerosis
OwnerDepei Liu, Zhigang She +3inventors
InventorsDepei Liu, Zhigang She, Wei Zheng, Yusheng Wei, Houzao Chen
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Abstract
Use of PON gene cluster in preparing medicament for treating atherosclerosis in mammals, wherein the PON gene cluster treat atherosclerosis by promoting stability of atherosclerotic plaque. Method for the developing PON gene cluster transgenic mouse model and use of PON gene cluster in the development of PON gene cluster positive transgenic mouse model with atherosclerosis are also provided.
Claims11
Use of PON gene cluster in preparing the medicament for treating atherosclerosis in mammals.
A method for the developing a PON gene cluster transgenic animal model, comprising the following steps of:
- a) linearizing a vector comprising human PON gene cluster with an appropriate restrictive endonuclease;
- collecting and treating the vector DNA by conventional method for use in microinjection;
- b) diluting the said DNA to approximate 1-2 ng/μL with buffer for microinjection and then microinjecting the diluted DNA into the fertilized egg of the animal surrogate;
- c) placing the fertilized egg in M16 medium after being micro-injected, incubating the fertilized egg at 37° C. for 1-2 days;
- d) transferring the fertilized egg of step c) to pseudo-pregnant animal surrogate, selecting PON gene cluster positive animals by PCR and Southern Blot Analysis after the newborn animals were delivered.
Use of PON gene cluster in the development of PON gene cluster positive transgenic mice models with atherosclerosis.
Dependencies are inferred from the claim text.