Method and system to detect and diagnose alzheimer's disease
OwnerPaul D. Colemaninventor
Life of this patent
Estimating the term…
Abstract
Various embodiments provide methods for the detection, the diagnosis, and/or the prediction of disease onset of Alzheimer's disease. Methods for determining a state of Alzheimer's disease are provided. Accordingly, these methods can comprise the steps of placing a sample comprising at least one blood component onto a substrate, labeling the sample to identify at least one epigenetic marker and at least one of an inflammation marker and a cellular stress marker; determining an amount of the markers; performing a multivariate statistical analysis to produce an output value; comparing the output value to a reference value; and determining a state of Alzheimer's disease.
Claims28
A method for determining a state of Alzheimer's disease in a patient, the method comprising:
- obtaining a biological sample comprising at least one blood component from the patient;
- determining an amount of expression for at least one epigenetic marker and at least one of an inflammation marker and a cellular stress marker in the biological sample;
- applying a multivariate statistical analysis to the amount of expression to produce an output value;
- comparing the output value to a reference value;
- and
- determining a state of Alzheimer's disease in the patient.
A method for determining the probability of developing Alzheimer's disease in a patient before the onset of symptoms, the method comprising:
- obtaining a biological sample comprising at least one blood component from the patient;
- determining an expression level of at least one epigenetic marker and at least one of an inflammation marker and a cellular stress marker;
- applying a multivariate statistical analysis to the expression level of the at least one epigenetic marker and the at least one of the inflammation marker and the cellular stress marker to produce an output value;
- comparing the output value to a reference value;
- wherein comparing the output value to the reference value results in a risk score;
- and
- determining the patient's risk of developing Alzheimer's disease based on the risk score.
A system for determining a state of Alzheimer's disease in a sample, the system comprising:
- a substrate comprising a top surface and a bottom surface;
- at least one first detail and at least one second detail on the top surface of the substrate;
- at least one first label operative to bind to at least one epigenetic marker in a sample comprising leukocyte, the at least one first label located in the at least one first detail;
- at least one second label operative to bind to at least one of an inflammation marker and a cellular stress marker in a sample comprising leukocyte, the at least one label located in the at least one second detail;
- a first reference value comprising a known amount of the at least one epigenetic marker;
- and
- a second reference value comprising a known amount of the at least one of the inflammation marker and the cellular stress marker.
Dependencies are inferred from the claim text.