Method and apparatus for detecting analytes

An apparatus for detecting analytes comprising:

• a tube;
• a selective filter that is placed at the bottom of the tube and that filters complexes that are coupled with the analytes and passes complexes that are not coupled with the analytes, in the case of inputting a sample containing the analytes and receptors made of complexes that are formed by coupling fluorescent-magnetic nanoparticles with antibodies, respectively;
• a magnet that is placed in the inside or outside of the tube to thus selectively attach the complexes to the wall of the tube;
• and
• a fluorescence measuring probe that is selectively inserted into the tube and measures fluorescence emitted from the complex coupled with the analyte and remaining in the filter, to thereby determine the analyte.

An apparatus for detecting analytes comprising:

• a tube;
• a selective filter that is placed at the bottom of the tube and that filters complexes that have been coupled with the analytes and passes free complexes that have not been coupled with the analytes, in the case that a sample containing the analytes and a receptor made of a complex that is formed by coupling fluorescent-magnetic nanoparticles with antibodies, respectively;
• a moving magnet that is placed in the inside of the tube and measures fluorescence, and that has a separator on the lower end of a magnet housing, in which the moving magnet having a first throughhole into which a penetration tube for sucking a sample except for the complexes that have been coupled with the analytes and the free complexes that have not been coupled with the analytes and supplying a buffer solution and a second throughhole into which a fluorescence measuring probe is inserted, is movably incorporated in the magnet housing;
• and
• the fluorescence measuring probe that is inserted into the second throughhole and that measures fluorescence emitted from the complex coupled with the analyte and remaining in the filter, to thereby determine the analyte.

An apparatus for detecting analytes comprising:

• an ion-exchange filter that selectively adsorbs only analyte-receptor complexes and passes free receptors that have not been coupled with the analytes, by using an isoelectric point or a difference in charges between the analyte-receptor complexes and the free receptors that have not been coupled with the analytes, when a sample containing the analyte-receptor complexes and the free receptors that have not been coupled with the analytes is supplied;
• a 3-way valve that is connected at the rear end of the ion-exchange filter and that separates the free receptors that have not been coupled with the sequentially input analytes from the analyte-receptor complexes;
• and
• a bio-sensor chip that detects the analyte-receptor complexes, wherein the 3-way valve comprises:
• a housing having a first port through which the free receptors that have not been coupled with the analytes and the analyte-receptor complexes are supplied, a second port through which the free receptors that have not been coupled with the analytes are discharged, and a third port through which the analyte-receptor complexes are discharged to the bin-sensor chip;
• and
• a rotating body that is rotatably provided in the housing, and comprises an internal passageway that are connected to first and second inlets, in which the first and second inlets are respectively matched to the first and second ports at an initial state and the first and second inlets are respectively matched to the third and first ports at a rotating state.

An apparatus for detecting analytes comprising:

• a 3-way valve that separates analyte-receptor complexes and free receptors that have not been coupled with analytes;
• and
• a bio sensor chip that detects the analyte-receptor complexes, wherein the 3-way valve comprises:
• a housing having a first port through which a sample containing the free receptors that have not been coupled with the analytes and the analyte-receptor complexes is supplied, a second port through which the free receptors that have not been coupled with the analytes are discharged, and a third port through which the an complexes are discharged to the bio-sensor chip;
• a rotating body that is rotatably provided in the housing, and comprises an internal passageway that is connected to first and second inlets, in which the first and second inlets are respectively matched to the first and second ports at an initial state and the first and second inlets are respectively matched to the third and first ports at a rotating state;
• and
• a micro-filter that is provided in the internal passageway between the first and second inlets and filters the analyte-receptor complexes and passes the free receptors that have not been coupled with the analytes.