Primers and Probes for the Amplification and Detection of HIV GAG, REV and NEF Polynucleotides

The composition of claim 12, which comprises the Gag R1833 primers of SEQ ID NO:42, SEQ ID NO:43 and SEQ ID NO:44.

The composition of claim 12, which comprises Gag R 1884 primers of SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47 and SEQ ID NO:48.

The composition of claim 12, further comprising one or more Gag forward primers selected from the group consisting of SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32 and SEQ ID NO:33.

The composition of claim 15, which comprises:

• a. the Gag R1884 reverse primers of SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47 and SEQ ID NO:48;
• and
• b. the Gag F124 primer of SEQ ID NO:30.

The composition of claim 15, which comprises:

• a. the Gag R1884 reverse primers of SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47 and SEQ ID NO:48;
• and
• b. the Gag F304 primer of SEQ ID NO:31.

The composition of claim 15, which comprises:

• a. the Gag R1884 reverse primers of SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47 and SEQ ID NO:48;
• and
• b. the Gag F334 primers of SEQ ID NO:32 and SEQ ID NO:33.

A method for amplifying an HIV Gag nucleic acid, comprising carrying out a polymerase chain reaction using at least one or a combination of Gag forward primers selected from the group consisting of SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32 and SEQ ID NO:33;

• and at least one or a combination of Gag reverse primers selected from the group consisting SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47 and SEQ ID NO:48.