Gene Expression Technique
AssigneeNovozymes Biopharma DK A/S
InventorsDarrell Sleep, Gillian Shuttleworth, Christopher John Arthur Finnis
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Abstract
The present disclosure relates to a method for producing heterologous protein including: (c) providing a host cell comprising a 2 μm-family plasmid, the plasmid comprising a gene encoding a protein comprising the sequence of a chaperone protein and a gene encoding a heterologous protein; (d) culturing the host cell in a culture medium under conditions that allow the expression of the gene encoding the chaperone protein and the gene encoding a heterologous protein; (e) purifying the thus expressed heterologous protein from the cultured host cell or the culture medium.
Claims24
A method for producing non-2 μm-family plasmid protein comprising:
- (a) providing a host cell comprising a 2 μm-family plasmid, the plasmid comprising a gene encoding protein comprising the sequence of a chaperone protein and a gene encoding a non-2 μm-family plasmid protein;
- (b) culturing the host cell in a culture medium under conditions that allow the co-expression of the gene encoding protein comprising the sequence of the chaperone protein and the gene encoding a non-2 μm-family plasmid protein;
- and
- (c) purifying the thus expressed non-2 μm-family plasmid protein from the cultured host cell or the culture medium.
A method of using a 2 μm-family plasmid as an expression vector to increase the production of a fungal (preferably yeast) or vertebrate non-2 μm-family plasmid protein comprising providing a gene encoding the non-2 μm-family plasmid protein and a gene encoding a chaperone protein on the same 2 μm-family plasmid.
A 2 μm-family plasmid comprising a gene encoding a protein comprising the sequence of a chaperone protein and a gene encoding a non-2 μm-family plasmid protein, wherein if the plasmid is based on the 2 μm plasmid then it is a disintegration vector.
A host cell comprising a 2 μm-family plasmid, the plasmid comprising a gene encoding protein comprising the sequence of a chaperone protein and a gene encoding a non-2 μm-family plasmid protein.
Dependencies are inferred from the claim text.