OPTIMIZED Fc VARIANTS AND METHODS FOR THEIR GENERATION
AssigneeXencor, Inc.
InventorsGregory Alan Lazar, Arthur J. Chirino, Wei Dang, John Rudolph Desjarlais, Stephen Kohl Doberstein, Robert J. Hayes, Sher Bahadur Karki, Omid Vafa
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Abstract
The present invention relates to optimized Fc variants, methods for their generation, and antibodies and Fc fusions comprising optimized Fc variants.
Claims12
1-67. (canceled)
A polypeptide comprising a variant Fc domain as compared to a parent Fc domain, wherein said variant Fc domain comprises an amino acid substitution at a position selected from the group consisting of 234, 235, 239, 240, 241, 243, 244, 245, 247, 262, 263, 264, 265, 266, 267, 269, 296, 297, 298, 299, 313, 325, 326, 327, 328, 329, 330, 332, 333, and 334, wherein said variant Fc domain has altered binding to an FcγR receptor and wherein numbering is according to the EU index in Kabat et al.
A nucleic acid encoding a polypeptide comprising a variant Fc domain as compared to a parent Fc domain, wherein said variant Fc domain comprises an amino acid substitution at a position selected from the group consisting of 234, 235, 239, 240, 241, 243, 244, 245, 247, 262, 263, 264, 265, 266, 267, 269, 296, 297, 298, 299, 313, 325, 326, 327, 328, 329, 330, 332, 333, and 334, wherein said variant Fc domain has altered binding to an FcγR receptor and wherein numbering is according to the EU index in Kabat et al.
A host cell comprising a nucleic acid encoding a polypeptide comprising a variant Fc domain as compared to a parent Fc domain, wherein said variant Fc domain comprises an amino acid substitution at a position selected from the group consisting of 234, 235, 239, 240, 241, 243, 244, 245, 247, 262, 263, 264, 265, 266, 267, 269, 296, 297, 298, 299, 313, 325, 326, 327, 328, 329, 330, 332, 333, and 334, wherein said variant Fc domain has altered binding to an FcγR receptor and wherein numbering is according to the EU index in Kabat et al.
A method of making a polypeptide comprising:
- a) culturing a host cell comprising a nucleic acid encoding a polypeptide comprising a variant Fc domain as compared to a parent Fc domain, wherein said variant Fc domain comprises an amino acid substitution at a position selected from the group consisting of 234, 235, 239, 240, 241, 243, 244, 245, 247, 262, 263, 264, 265, 266, 267, 269, 296, 297, 298, 299, 313, 325, 326, 327, 328, 329, 330, 332, 333, and 334, wherein said variant Fc domain has altered binding to an FcγR receptor and wherein numbering is according to the EU index in Kabat et al., under conditions wherein said polypeptide is produced;
- and
- b) purifying said polypeptide.
Dependencies are inferred from the claim text.