Tissue transplant compositions and methods for use
AssigneesParcell Laboratories LLC, The Brigham and Women's Hospital, Inc.
InventorsKeith D. Crawford, Pamela Layton, John M. Garvey
Life of this patent
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Abstract
Provided are transplants and methods for augmenting formation and restoration of organ and tissue, for example, bone formation, by administering autologous or allogeneic human embryonic-like adult stem cells (ELA cells). Also provided is a method for augmenting formation of tissues and organs by administering a transplant having ELA stem cells or combination of ELA stem cells.
Claims24
-37. (canceled)
A transplant composition comprising:
- a T-cell- or NK-cell-suppressive amount of a non-expanded population of early lineage adult (ELA) stem cells, an immune cell population comprising plasmacytoid dendritic cells, and a cryopreservative, wherein the ELA stem cells express at least one of Oct4, Nanog and Sox2, and do not detectably express CD13, CD45, CD90 or CD34, and wherein the transplant composition is without detectable erythrocyte cells.
A transplant composition comprising:
- a T-cell- or NK cell-suppressive dose of a culture-expanded population of early lineage adult (ELA) stem cells characterized by expression of at least one of Oct4, Nanog and Sox2, without detectable expression of CD13, CD45, CD90 or CD34, wherein the transplant composition is without detectable erythrocyte cells or plasmacytoid dendritic cells, the composition further comprising sterile isotonic cell culture medium and a cryopreservative.
A method of treating a subject in need of an allograft transplant comprising:
- identifying a subject having a tissue in need of repair;
- and
- contacting the tissue of the subject with a transplant composition comprising a transplant composition comprising:
- a T-cell- or NK-cell-suppressive amount of a non-expanded population of early lineage adult (ELA) stem cells, an immune cell population comprising plasmacytoid dendritic cells, and a cryopreservative, wherein the ELA stem cells express at least one of Oct4, Nanog and Sox2, and do not detectably express CD13, CD45, CD90 or CD34, and wherein the transplant is without detectable erythrocyte cells, wherein the growth of new tissue in the subject treats the subject.
A kit comprising a transplant composition comprising a sterile, buffered, isotonic preparation having a T-cell- or NK-cell suppressive amount of a population of early lineage adult (ELA) stem cells, and a cryopreservative, wherein the ELA stem cells express at least one of Oct4, Nanog and Sox2, and do not detectably express CD13, CD45, CD90 or CD34, wherein the transplant is without detectable erythrocyte cells, the kit further comprising suitable aseptic packaging of the transplant and instructive indications for use of the transplant.
A method of treating a subject in need of an allograft transplant comprising:
- identifying a subject having a cutaneous tissue defect in a treatment site;
- engrafting to the tissue defect site of the subject an ELA stem cell transplant comprising a population of ELA stem cells and immune cells;
- and
- occluding the engrafted tissue defect site with at least one of:
- composite allografts, biosynthetic dressings, acellular dermal allografts, autografts or cultured skin substitutes, and skin allografts or xenografts, wherein the transplant induces growth of new tissue including one or more of:
- hypodermal, dermal, and epidermal tissues of skin, connective tissue, sebaceous, vascular endothelial, cardiac muscle and neural tissues at the site, and does not induce an immune response in the subject against the transplant or resultant tissue, and wherein the growth of tissue in the subject thereby treats the subject.
A transplant composition comprising:
- a T-cell- or NK cell-suppressive dose of clonally-derived, culture-expanded, early lineage adult (ELA) stem cells characterized by expressing at least one of Oct4, Nanog and Sox2, without detectable expression of CD13, CD45, CD90 or CD34 and being without detectable quantities of erythrocyte cells or plasmacytoid dendritic cells, the transplant composition further comprising sterile isotonic cell culture medium and a cryopreservative.
A clonally derived population of cells originating from a substantially purified, isolated population of ELA cells from a single donor source.
Dependencies are inferred from the claim text.