The isolation and characterisation of heparan sulphates and their use in pharmaceutical compositions, methods of treatment and stem cell culture media suitable for conditions associated with bone repair
AssigneeAgency for Science, Technology and Research
InventorsSimon Cool, Victor Nurcombe, Murali Sadasivam
Life of this patent
Estimating the term…
Abstract
Heparan sulphates isolated from male and female murine liver, their characterisation and use in pharmaceutical compositions, methods of treatment and in stem cell culture media suitable for conditions associated with bone repair.
Claims23
1-17. (canceled)
A method of treating a bone fracture in a patient, the method comprising administration of a therapeutically effective amount of heparan sulphate MML HS to the patient wherein the MML HS has N-sulfation of between about 14% and about 22%.
A method of treating a bone fracture in a patient, the method comprising surgically implanting a biocompatible implant or prosthesis, which implant or prosthesis comprises a biomaterial and MML HS, into tissue of the patient at or surrounding the site of fracture, wherein the MML HS has N-sulfation of between about 14% and about 22%.
23-33. (canceled)
A method for the repair, replacement or regeneration of bone tissue in a human or animal patient in need of such treatment, the method comprising:
- (i) culturing mesenchymal stem cells in vitro in contact with MML HS for a period of time sufficient for said cells to form bone tissue or bone precursor cells, wherein the MML HS has N-sulfation of between about 14% and about 22%.;
- (ii) collecting said bone tissue or bone precursor cells;
- and
- (iii) implanting said bone tissue or bone precursor cells into the body of the patient at a site of injury or disease to repair, replace or regenerate bone tissue in the patient.
35-74. (canceled)
Dependencies are inferred from the claim text.