Protein nanoparticle dispersions
AssigneeBoard of Regents, The University of Texas System
InventorsKeith P. Johnston, Jennifer A. Maynard, Andrea Miller, Brian Wilson, Thomas M. Truskett, Ameya Borwankar, Aileen Dinin
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Abstract
Provided herein, inter alia, are protein dispersions comprising dense protein nanoclusters and methods of making the. Upon dilution, the clusters may reversibly dissociate into native protein molecules with high biological activity. The viscosities of the nanocluster dispersions may be sufficiently low to allow small-volume subcutaneous injections.
Claims20
A transparent, low viscosity, high protein concentration dispersion, wherein said dispersion comprises a plurality of nanoclusters, wherein each of said plurality of nanoclusters comprises a plurality of proteins, wherein each of said plurality of proteins shares amino acid sequence identity.
A method of making a transparent, low viscosity, high protein dispersion of protein nanoclusters comprising concentrating a protein-crowder liquid combination and thereby forming said dispersion, wherein said dispersion comprises a plurality of nanoclusters, wherein each of said plurality of nanoclusters comprises a plurality of proteins, wherein each of said plurality of proteins shares amino acid sequence identity;
- wherein said dispersion is a transparent, low viscosity, dispersion;
- wherein said dispersion comprises a concentration of said protein of greater than about 200 mg/mL, and wherein said dispersion comprises a plurality of a crowder.
A method of making a transparent, low viscosity, high protein dispersion of protein nanoclusters comprising the step of combining a protein in powder form with a crowder and a dispersion liquid thereby forming a dispersion comprising a plurality of nanoclusters comprising a plurality of said protein, wherein each of said plurality of proteins shares amino acid sequence identity;
- wherein said dispersion is a transparent, low viscosity, dispersion;
- wherein said dispersion comprises a concentration of said protein of greater than about 200 mg/mL.
A method of making a transparent, low viscosity, high protein dispersion of protein nanoclusters comprising the step of combining a protein in powder form with a dispersion liquid thereby forming a dispersion comprising a plurality of nanoclusters comprising a plurality of said protein, wherein each of said plurality of proteins shares amino acid sequence identity;
- wherein said dispersion is a transparent, low viscosity, dispersion;
- wherein said dispersion comprises a concentration of said protein of greater than about 200 mg/mL.
A method of modifying the average protein nanocluster diameter of a transparent, low viscosity, high protein dispersion of protein nanoclusters comprising increasing or decreasing the concentration of a crowder or said protein in said dispersion, wherein said dispersion comprises a plurality of nanoclusters, wherein each of said plurality of nanoclusters comprises a plurality of proteins, wherein each of said plurality of proteins shares amino acid sequence identity;
- wherein said dispersion is a transparent, low viscosity, dispersion;
- and wherein said dispersion comprises a concentration of said protein of greater than about 200 mg/mL.
A kit, wherein the kit comprises a protein in powder form or a protein-crowder mixture in powder form, and a dispersion liquid.
Dependencies are inferred from the claim text.