Enhancer for promoter, and use thereof
AssigneeThe Research Foundation for Microbial Diseases of Osaka University
InventorsMasaaki Matsuura, Masaya Takemoto, Tetsuo Koshizuka, Koichi Yamanishi, Yasuko Mori
Life of this patent
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Abstract
Disclosed is an enhancer for a viral promoter such as a promoter that can induce expression selectively and strongly in immunocompetent cells (e.g., lymphocytes) or blood cells. It is found unexpectedly that an intron has the above-mentioned enhancer activity. Thus, it is found that an enhancer for a promoter, which comprises an intron sequence for a major immediate early gene (MIE) of human herpes virus-6 (HHV-6) (particularly HHV-6B) or a fragment of the intron sequence, has a potent promoter activity.
Claims14
An enhancer for a promoter, comprising:
- an intron sequence for a major immediate early gene (MIE) of human herpes virus-6 (HHV-6) (hereinafter, referred to as HHV-6B) or a fragment of the intron sequence.
A promoter with improved activity, comprising:
- an intron sequence for a major immediate early gene (MIE) of human herpes virus-6 (HHV-6) or a fragment of the sequence;
- and a promoter.
A construct for enhancing expression of a gene in a cell, comprising:
- an intron sequence for a major immediate early gene (MIE) of human herpes virus-6 (HHV-6) or a fragment of the sequence;
- and a promoter.
Use of a sequence (SEQ ID No. 2) of −12 to +1292 based on a transcription initiation point of IE1 included in the 6MIE sequence, or a fragment of the sequence, as an enhancer for a promoter.
Dependencies are inferred from the claim text.