Bacteriophage T4 Nanoparticles for Eukaryotic Delivery
AssigneeThe Government of the United States of America, as represented by the Secretary of the Navy
InventorsJinny Lin Liu, Kelly L. Robertson, Carissa M. Soto
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Abstract
A T4 nanoparticle is a non-infectious, tail-less variant of a T4 bacteriophage. In one embodiment, eukaryotic cells are labeled with dyed T4 nanoparticles, wherein each dyed T4 nanoparticle comprises at least 350 dye molecules covalently bound thereto. In another embodiment, T4 nanoparticles are used to deliver exogenous DNA to eukaryotic cells for protein expression therein. It is contemplated that T4 nanoparticles may be used to deliver other exogenous material to eukaryotic cells.
Claims15
A method of labeling eukaryotic cells, the method comprising:
- providing dyed T4 nanoparticles, wherein each dyed T4 nanoparticle comprises at least 350 dye molecules covalently bound thereto, and
- contacting eukaryotic cells with the dyed T4 nanoparticles to obtain labeled eukaryotic cells.
A method of inducing protein expression in eukaryotic cells, the method comprising:
- providing T4 nanoparticles comprising exogenous DNA encoding a protein, and
- contacting eukaryotic cells with the T4 nanoparticles thereby resulting in expression of the protein in the eukaryotic cells.
A method of delivering material to eukaryotic cells, the method comprising:
- providing T4 nanoparticles comprising an exogenous material and
- contacting eukaryotic cells with the T4 nanoparticles to obtain eukaryotic cells comprising the exogenous material.
Dependencies are inferred from the claim text.