Alteration of cell membrane for new functions
AssigneeUniversity of Louisville Research Foundation, Inc.
InventorHaval Shirwan
Life of this patent
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Abstract
Methods and compositions are provided for the persistent modification of cell membranes with exogenous proteins so as to alter the function of the cell to achieve effects similar to those of gene therapy, without the introduction of exogenous DNA. DNA sequences, the proteins and polypeptides embodying these sequences are disclosed for modulating the immune system. The modulations include down-regulation, up-regulation and apoptosis.
Claims20
1-20. (canceled)
A method of attaching an immunomodulatory agent to a cell surface, the method comprising:
- contacting a biotinylated cell with a chimeric protein comprising (i) the immunomodulatory agent and (ii) avidin or streptavidin, wherein the immunomodulatory agent is selected from the group consisting of TGF-β, CD40L, and IL-2.
An isolated nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NOs. 3, 4 and 6.
Dependencies are inferred from the claim text.