Production of an intact virus in a mammalian (non-host) cell system using a secondary non-host viral construct
OwnerArun K. Dharinventor
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Abstract
The present invention relates to constructs and methods for the production of recombinant proteins, viruses, and viral vaccines in heterologous culture systems by expressing intact genes or viral genomes under the control of a pantropic promoter in a culture system that is not considered a host to the virus so produced. The promoter/viral genome constructs are inserted into a baculovirus and expressed in mammalian non-host cells for the baculovirus.
Claims16
A viral construct comprising a carrier virus, at least one viral promoter of Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) operably linked to at least one inclusion viral genome, gene or fragment thereof, wherein the inclusion viral genome is different from that of the carrier virus.
A method to produce a functional virus in a heterologous system, the method comprising:
- preparing a nucleotide sequence of a virus;
- linking a IHHNV promoter sequence upstream of the 5′ end of the nucleotide sequence to drive the transcription of the nucleotide sequence;
- inserting the linked sequences into a transfecting carrier virus;
- and
- infecting and culturing a host cell for expression therein of the linked sequences and carrier virus.
A method of expressing recombinant proteins in a heterologous culture system, the method comprising:
- preparing a nucleotide sequence encoding the proteins,
- linking a IHHNV promoter sequence upstream of the 5′ end of the nucleotide sequence encoding the proteins to drive the transcription of the nucleotide sequence and forming a linked sequence;
- inserting the linked sequence into a transfecting carrier virus;
- and
- infecting and culturing a mammalian host cell for expression therein of the proteins and wherein the carrier virus is not expressed.
Dependencies are inferred from the claim text.