Methods for the synthesis of polyadenylic acid
AssigneeIbis Biosciences, Inc.
InventorsMark W. Eshoo, Curtis Phillipson
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Abstract
The present invention provides compositions, kits, and methods for synthesizing polyadenylic acid using polynucleotide phosphorylase, adenosine diphosphate, a buffering agent, and a divalent metal cation. In certain embodiments, the adenosine diphosphate is present at a concentration between about 5.0 mM and about 100 mM, and the buffering agent has a pH around 8.0. In some embodiments, the prepared polyadenylic acid compositions are free of detectable nucleic acid.
Claims20
A composition comprising or consisting essentially of:
- a) polynucleotide phosphorylase;
- b) adenosine diphosphate (ADP) present at a concentration between 5.0 mM and 100 mM;
- c) a buffering agent with a pH between 7.3 and 8.7;
- and
- d) a divalent metal cation.
A method of making polyadenylic acid comprising:
- a) combining polynucleotide phosphorylase, adenosine diphosphate (ADP), a buffering agent with pH between 7.4 and 8.6, and a divalent metal cation, to generate a mixture, wherein said ADP is present in said mixture at a concentration between 5.0 mM and 100 mM;
- and
- b) incubating said mixture under conditions such that a composition comprising polyadenylic acid is generated.
A system comprising, or consisting essentially of, or consisting of, the following components:
- a) polynucleotide phosphorylase;
- b) adenosine diphosphate (ADP) present at a concentration between 5.0 mM and 100 mM;
- c) a buffering agent with a pH between 7.4 and 8.6;
- and
- d) a divalent metal cation.
Dependencies are inferred from the claim text.