Methods and compositions for producing linear alkyl benzenes
AssigneeLS9, Inc.
InventorsMathew A. RUDE, Andreas W. SCHIRMER
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Abstract
Compositions and methods for producing hydrocarbons using recombinant cells are described herein. Also described herein are recombinant cells, recombinant cell cultures and methods for producing linear alkyl benzenes (LABs) using hydrocarbons produced by such recombinant cell cultures.
Claims19
A recombinant host cell culture for production of a linear alkene or alkane, the host cell culture comprising a recombinant microorganism engineered to express a polynucleotide encoding a polypeptide having the amino acid sequence presented as SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, or 36, wherein a linear alkene or alkane is found in the cell-free culture supernatant.
A method of producing a linear alkyl benzene, the method comprising:
- (i) expressing in a host cell a polynucleotide comprising the nucleotide sequence of SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35,
- (ii) culturing the host cell in the presence of a carbon source, thereby producing a linear alkene;
- (iii) isolating the linear alkene from the host cell culture;
- and
- (iv) reacting the linear alkene with benzene in the presence of a catalyst under reaction conditions sufficient for alkylation of the benzene, thereby producing a linear alkyl benzene.
Dependencies are inferred from the claim text.