Recombinant host cells comprising phosphoketolases
AssigneeButamax(Tm) Advanced Biofuels LLC
InventorsMichael Dauner, Lori Ann Maggio-Hall, Jean-Francois Tomb
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Abstract
The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.
Claims35
A recombinant host cell comprising:
- (i) at least one deletion, mutation, or substitution, or combinations thereof, in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA;
- and
- (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity.
A recombinant host cell comprising:
- (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity;
- and
- (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity.
A method of producing a recombinant host cell comprising:
- transforming a host cell comprising at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity with a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity.
A method of improving the growth of a recombinant host cell comprising at least one deletion, mutation or substitution in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity, comprising:
- transforming said recombinant host cell with a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity.
A method of reducing the requirement for an exogenous two-carbon substrate for the growth of a recombinant host cell comprising at least one deletion, mutation or substitution in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity, comprising:
- transforming said recombinant host cell with a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity.
Dependencies are inferred from the claim text.