Immunoassay for thrombin detection
AssigneeOmrix Biopharmaceuticals Ltd.
InventorsRoni Mintz, Nadav Orr, Israel Nur
Life of this patent
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Abstract
The invention relates to an in vitro immunoassay for quantifying thrombin in a sample comprising anti-thrombin III (AT-III) and thrombin. The method comprises the following steps: contacting the sample with a small molecule that recognizes the substrate binding site of thrombin; contacting the thrombin with a thrombin specific antibody raised against a thrombin blocked in the active site; and measuring the level of bound antibody.
Claims20
An in vitro immunoassay for quantifying thrombin in a sample in the presence of anti-thrombin III (AT-III), the immunoassay comprising the steps of:
- contacting the sample with a small molecule that recognizes the substrate binding site of thrombin;
- adding to the sample a thrombin specific antibody, wherein the thrombin specific antibody is raised against a thrombin having a blocked active site;
- measuring the binding of the antibody to the thrombin;
- and determining the amount of thrombin present.
A kit for quantifying thrombin in a sample comprising anti-thrombin III (AT-III), the kit comprises a thrombin specific polyclonal antibody or a fragment thereof and a small molecule that recognizes the substrate binding site of thrombin, wherein the thrombin specific antibody is raised against a thrombin having a blocked active site.
An in vitro immunoassay for quantifying thrombin in a sample comprising anti-thrombin III (AT-III) and thrombin, the immunoassay comprises the steps of:
- contacting the sample with a small molecule that recognizes the substrate binding site of thrombin;
- contacting the thrombin with a thrombin specific antibody raised against a thrombin blocked in the active site;
- and measuring the level of bound antibody.
Dependencies are inferred from the claim text.