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US 2012/0142547 A1Published application
Polypeptide immobilization
AssigneeThe University of Chicago
InventorsMilan Mrksich, Christian Hodneland
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Abstract
The present invention provides a method, comprising (a) providing a reactant ligand attached to a substrate; (b) contacting the substrate with a fusion polypeptide, said fusion polypeptide comprising a capture polypeptide fused to a display polypeptide under conditions such that said reactant ligand covalently binds to said capture polypeptide; and (c) analyzing said display polypeptide.
Claims15
A method, comprising:
- a) providing a reactant ligand attached to a substrate;
- b) contacting the substrate with a fusion polypeptide, said fusion polypeptide comprising a capture polypeptide fused to a display polypeptide under conditions such that said reactant ligand covalently binds to said capture polypeptide;
- and
- c) analyzing said display polypeptide.
1 more element
A method of analyzing a protein chip, comprising:
- a) providing a protein chip, said protein chip comprising:
- a substrate;
- and a reaction product of a reactant ligand covalently bonded to a fusion polypeptide, the reactant ligand attached to said substrate;
- wherein said fusion polypeptide comprises a capture polypeptide moiety corresponding to said reactant ligand;
- and
- b) analyzing said fusion polypeptide attached to said protein chip.
3 more elements
Dependencies are inferred from the claim text.