Foamyvirus vectors and methods of use
OwnerAxel Rethwilm, Dirk Lindemann +2inventors
InventorsAxel Rethwilm, Dirk Lindemann, Helmut Hanenberg, Wade D. Clapp
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Abstract
The disclosure of the present application provides foamyvirus vectors, as well as methods and kits involving the same. In at least one embodiment, a plurality of recombinant vectors comprises an expression sequence encoding at least one component of a foamyvirus particle, wherein at least one codon of the expression sequence is optimized for expression in homo sapiens.
Claims34
A foamyvirus expression system, the system comprising:
- a plurality of recombinant vectors comprising an expression sequence encoding at least one component of a foamyvirus particle, wherein the expression sequence is optimized for expression in homo sapiens.
A foamyvirus expression system comprising a plurality of recombinant vectors, the plurality of recombinant vectors comprising:
- a first nucleotide sequence having at least a 75% nucleotide identity to SEQ. ID. NOS. 1;
- a second nucleotide sequence having at least a 75% nucleotide identity to SEQ. ID. NOS. 2, and
- a second nucleotide sequence having at least a 75% nucleotide identity to SEQ. ID. NOS. 3 or 4;
- wherein the plurality of recombinant vectors are capable of producing a foamyvirus particle when transformed into an appropriate host cell.
A kit for the expression of a foamyvirus in mammalian cells, the kit comprising;
- a plurality of recombinant vectors, the plurality of recombinant vectors comprise a foamyvirus expression sequence, a first vector, a second vector, a third vector, and a fourth vector;
- wherein the foamyvirus expression sequence is optimized for expression in homo sapiens;
- wherein the first vector comprises a viral packaging signal, the second vector comprises a sequence encoding gag, the third vector comprises a sequence encoding pol, and the fourth vector comprises a sequence encoding env;
- wherein the concentration of the first vector is greater than the second vector;
- the concentration of the second vector is greater than the third vector;
- the concentration of the third vector is greater than the fourth;
- wherein the plurality of recombinant vectors produces foamyvirus particles when transfected into mammalian cells.
The kit of claim 31, wherein a ratio of first vector to second vector to third vector to fourth vector is about 10 to about 5 to about 1 to about 1 respectively.
A method of use of a foamyvirus expression system, the method comprising:
- introducing a plurality of recombinant vectors having an optimized foamyvirus expression sequence into a host cell;
- introducing the host cell containing the plurality of recombinant vectors into a host organism;
- wherein the plurality of recombinant vectors is operable to produce foamyvirus particles after introduction into the host cell.
Dependencies are inferred from the claim text.