Backflush methods and devices for chromatography
AssigneePerkinElmer Health Sciences, Inc.
InventorAndrew Tipler
Life of this patent
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Abstract
Certain embodiments described herein are directed to chromatography systems that include a microfluidic device and that implement one or more methods to direct sample to a desired fluid flow path. The methods can be used to backflush a sample to a desired fluid flow path to select certain analytes within a sample.
Claims19
A method of modulating flow of a fluid in a chromatography system including a microfluidic device fluidically coupled to an injector, the method comprising:
- controlling fluid pressure of the system to permit a sample to flow from the injector and into the microfluidic device and to a first fluid flow path and a second fluid flow path each fluidically coupled to the microfluidic device at a separate outlet port of the microfluidic device;
- and
- altering the pressure of the system to reverse the flow of the sample in at least one of the first fluid flow path and the second fluid flow path to backflush sample in the fluid flow path where the pressure is reversed.
A system comprising:
- a microfluidic device comprising an input port and at least two outlet ports each fluidically coupled to the inlet port;
- a first fluid flow path fluidically coupled to one outlet port of the microfluidic device;
- a second fluid flow path fluidically coupled to the other outlet port of the microfluidic device;
- a first restrictor in the first fluid flow path, the first restrictor configured to be fluidically coupled to a first pressure source;
- and
- a second restrictor in the second fluid flow path, the second restrictor configured to be fluidically coupled to a second pressure source.
A method of backflushing column effluent in a chromatography system including two different fluid flow paths each fluidically coupled to a microfluidic device, the method comprising altering the pressure in one of the fluid flow paths to backflush column effluent in that fluid flow path to the other fluid flow path.
19-24. (canceled)
Dependencies are inferred from the claim text.