Novel Monolignol 4-O-Methyltransferases and Uses Thereof
AssigneeBrookhaven Science Associates, LLC
InventorsChang-Jun Liu, Mohammad-Wadud Bhuiya, Kewei Zhang
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Abstract
Modified (iso)eugenol 4-O-methyltransferase enzymes having novel capacity for methylation of monolignols and reduction of lignin polymerization in plant cell wall are disclosed. Sequences encoding the modified enzymes are disclosed.
Claims37
A modified (iso)eugenol 4-O-methyltransferase (IEMT) enzyme having at least one amino acid substitution at residues selected from the group consisting of residues 130, 131, 133, 134, 139, 164, 165, 175, 186, 219, 326 or 327, where the amino acid residues correspond to those identified in GenBank U86760.1.
An isolated nucleic acid sequence encoding an (iso)eugenol methyltransferase enzyme having at least one amino acid substitution at residues selected from the group consisting of residues 130, 131, 133, 134, 139, 164, 165, 175, 186, 219, 326 or 327.
A modified (iso)eugenol 4-O-methyltransferase (IEMT) enzyme comprising an IEMT enzyme having at least one amino acid substitution at residues selected from the group consisting of residues 130, 131, 133, 134, 138, 139, 157, 161, 164, 165, 166, 168, 169, 173, 175, 179, 183, 186, 219, 319, 322, 326 or 327, where the amino acid residues correspond to those identified in GenBank U86760.1.
A modified (iso)eugenol 4-O-methyltransferase (m-IEMT) enzyme having an increased activity compared to a wild type (iso)eugenol 4-O-methyltransferase (wt-IEMT) for 4-O-methylation of coniferyl alcohol or sinapyl alcohol or both.
A modified (iso)eugenol 4-O-methyltransferase (m-IEMT) having an increased, compared to wild type (iso)eugenol 4-O-methyltransferase (wt-IEMT) for 4-O-methylation of coniferyl alcohol or sinapyl alcohol or both, said m-IEMT having one or more amino acid substitutions at residues 133, 165, 166, 169 and 175, where amino acid residues correspond to those identified in GenBank U86760.1.
Dependencies are inferred from the claim text.