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US 2012/0107181 A1Published application
Apparatus for synthesizing oligonucleotides and methods of use
AssigneeIsis Pharmaceuticals, Inc.
InventorsIsaiah E. Cedillo, Max N. Moore, Francis J. Ring
Life of this patent
Estimating the term…
Abstract
The present invention relates to apparatus and methods for synthesizing oligonucleotides wherein a reaction zone has a variable volume based on the position of a piston, and the piston is adjusted to control the amount of headspace above solid support in the reaction zone. Methods and apparatus that limit the size or existence of the headspace are described.
Claims30
A reactor for synthesizing oligonucleotides comprising:
- a vessel configured to contain a solid support, the vessel having an inlet and an outlet;
- a piston operatively arranged to reciprocate with the vessel;
- an actuator coupled to the piston;
- and
- a monitor configured to at least periodically adjust the actuator during a synthesis process to maintain the piston substantially in contact with the solid support.
2 more elements
A synthesizer comprising:
- a vessel configured to contain a granular polymeric material, the vessel having an inlet and an outlet, the vessel further configured to receive a fluid through the inlet;
- a piston operatively arranged to reciprocate with the vessel;
- an actuator coupled to the piston;
- and
- a monitor configured to at least periodically adjust the actuator during a synthesis process to maintain the piston within a predetermined distance from the granular polymeric material.
2 more elements
A synthesizer comprising:
- a vessel defining a reaction zone that accepts a predetermined volume of solid support, the vessel having an inlet and an outlet;
- a piston operatively arranged to reciprocate with the vessel;
- an actuator coupled to the piston;
- and
- a monitor configured to at least periodically adjust the actuator and piston to accommodate changes in the volume of the solid support during a synthesis process.
2 more elements
Dependencies are inferred from the claim text.