Silane mixtures
AssigneeAffymetrix, Inc.
InventorsGlenn H. McGall, Robert G. Kuimelis
Life of this patent
Estimating the term…
Abstract
Silanation compositions containing a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis are useful in modulating the active site density and hydrolytic stability of a surface. These compositions are particularly useful in silanating a surface prior to preparation of a polymer array and provide for increased hybridization results.
Claims19
A method of preparing an array of polymers comprising:
- providing a substrate;
- contacting a silica or other silicon oxide-containing surface of the substrate with a silanation composition that comprises a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis, wherein the molar ratio of the at least one silanation reagent which includes no functional group capable of supporting polymer synthesis and the at least one silanation reagent which includes a functional group capable of supporting polymer synthesis are present at ranges from about 50 to 1 to 500 to 1, respectively;
- protecting the functional group capable of supporting polymer synthesis with a protecting group or reacting the functional group capable of supporting polymer synthesis with a compound having a reactive group protected by a protecting group;
- removing the protecting group in selected regions of the surface to provide an exposed functional or reactive group;
- reacting said exposed group with a monomer, wherein the monomer is coupled to the exposed group;
- and
- repeating the steps of removing and reacting to produce the array of polymers.
9-11. (canceled)
15-16. (canceled)
A method of preparing an array of polymers according to claim 16 wherein the molar ratio of 1,2-bis(trimethoxysilyl)ethane to bis(2-hydroxyethyl)-3-aminopropyltriethoxysilane ranges from about 50 to 1 to 100 to 1, respectively.
An array of polymers on a surface of a substrate, where the surface of the substrate is silanated by a silanation composition that includes a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis, and the polymers of the array are covalently attached via this functional group to the silanated substrate, and at least one silanation reagent includes no functional group capable of supporting polymer synthesis, wherein the molar ratio of the at least one silanation reagent which includes no functional group capable of supporting polymer synthesis and the at least one silanation reagent which includes a functional group capable of supporting polymer synthesis are present at ranges from about 50 to 1 to 500 to 1, respectively.
A silanation composition comprising a mixture of two or more silanation reagents, wherein at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent which includes no functional group capable of supporting polymer synthesis, wherein the molar ratio of the at least one silanation reagent which includes no functional group capable of supporting polymer synthesis and the at least one silanation reagent which includes a functional group capable of supporting polymer synthesis are present at ranges from about 50 to 1 to 500 to 1, respectively.
Dependencies are inferred from the claim text.