Engineering salt tolerance in photosynthetic microorganisms
AssigneeSapphire Energy, Inc.
InventorsMichael Mendez, Su-Chiung Fang, Stephane Richard
Life of this patent
Estimating the term…
Abstract
Provided herein are compositions and methods for engineering salt tolerance and producing products by photosynthetic organisms. The photosynthetic organisms can be genetically modified to be salt tolerant as compared to an unmodified organism and to produce useful products. The methods and compositions of the disclosure are useful in many therapeutic and industrial applications.
Claims24
1-415. (canceled)
An isolated polynucleotide capable of transforming a photosynthetic organism, wherein the polynucleotide comprises a nucleic acid sequence of SEQ ID NO:
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- or a nucleic acid sequence comprising at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to a nucleic acid sequence of SEQ ID NO:
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- wherein the nucleic acid sequence encodes for a protein that when expressed results in a salt tolerant photosynthetic organism as compared to a photosynthetic organism that is not transformed by the isolated polynucleotide.
An isolated photosynthetic organism transformed with an exogenous polynucleotide, wherein the exogenous polynucleotide comprises at least one nucleic acid sequence encoding a protein that when expressed in the photosynthetic organism, results in the photosynthetic organism becoming a more salt tolerant photosynthetic organism as compared to a photosynthetic organism that is not transformed by the exogenous polynucleotide, and wherein the at least one nucleic sequence is SEQ ID NO:
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- or a nucleic acid sequence comprising at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to a nucleic acid sequence of SEQ ID NO:
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A method for increasing salt tolerance of a photosynthetic organism comprising, (a) transforming the photosynthetic organism with an exogenous polynucleotide sequence comprising a nucleic acid sequence, wherein the nucleic acid sequence encodes a protein that when expressed in the photosynthetic organism, results in increased salt tolerance of the photosynthetic organism as compared to a photosynthetic organism that is not transformed by the exogenous polynucleotide sequence, and wherein the nucleic acid sequence is SEQ ID NO:
- 55, SEQ ID NO:53, SEQ ID NO:
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- or a nucleic acid sequence comprising at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to a nucleic acid sequence of SEQ ID NO:
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Dependencies are inferred from the claim text.