# US 2012/0084015 A1 · Imaging Method and Use Thereof

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- Number: US 2012/0084015 A1
- Document ID: US20120084015A1
- Status: Published application
- Kind: A1
- Application number: 13/321,935
- Filed: 2010-05-28
- Published: 2012-04-05
- Earliest claimed: 2009-05-28
- Inventors: Christoph Riethmüller
- Web page: https://patentgrep.com/patent/US20120084015A1

## CPC

- G01N 33/5026: {on cell morphology}
- B82Y 15/00: Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
- B82Y 35/00: Methods or apparatus for measurement or analysis of nanostructures
- G01Q 30/04: Display or data processing devices
- G01Q 60/42: Functionalisation
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- G01Q: SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]

## Abstract

The present invention relates to a method based on atomic force microscopy and the use thereof on biological surfaces. A method is provided to detect the Local Deviational Volume (LDV) of defined subcellular structures irrespective of a biochemical characterisation

## Claims

### Claim 1

Method for determining and quantifying the topographical elements of biological surfaces using data received by atomic force microscopy, comprising the steps of
a. Preparing in vitro a single cell, cellular monolayer or tissue section;
b. Determining the local deviational volume (LDV) of subcelluar structures in a predefined mask in xy-plane;
c. Normalizing the positive or negative volume of the predefined area;
d. Quantifying the local deviational volume;
e. Analysing the data by comparing them with characteristic topographical elements of a calibrated sample;
f. Evaluating the quantified structural elements to obtain parameter sets.

### Claim at position 2

2-17. (canceled)