Polysaccharide transferase
AssigneeAdelaide Research & Innovation Pty Ltd
InventorsMaria Hrmova, Geoffrey Bruce Fincher
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Abstract
The present invention is predicated on the discovery that a polysaccharide transferase purified from barley seedlings can catalyze the formation of covalent bonds between different polysaccharides, including between xyloglucans and cellulose, and between xyloglucans and (1,3;1,4)-β-D-glucans. The present invention thus provides, among other things, an isolated or substantially purified polysaccharide transferase, wherein said polysaccharide transferase is capable of catalyzing the formation of a covalent bond between a donor polysaccharide and an acceptor polysaccharide; or a functionally active fragment or variant of said polysaccharide transferase.
Claims15
A method for modulating covalent bonding between a donor polysaccharide and an acceptor polysaccharide in a cell, the method comprising modulating the level or activity of a polysaccharide transferase in said cell, wherein the polysaccharide transferase comprises:
- (i) the amino acid sequence set forth in SEQ ID NO:
- 1;
- or
- (ii) an amino acid sequence which encodes a functionally active fragment or variant of a polysaccharide transferase referred to at (i), said fragment or variant comprising at least 60% amino acid sequence identity to SEQ ID NO:
- 1,
- wherein said polysaccharide transferase is capable of catalyzing the formation of a covalent bond between said donor polysaccharide and said acceptor polysaccharide, wherein at least one of said donor polysaccharide and said acceptor polysaccharide is a polysaccharide other than a xyloglucan, or said donor polysaccharide and said acceptor polysaccharide are of a different type.
Dependencies are inferred from the claim text.